Pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.

Ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. In this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. Serum, tissue chamber fluid (TCF), and bronchial secretion (BS) concentrations of ceftiofur were measured by microbiological assay after the first and fourth dose of each dosing regimen. Peak serum concentrations (Cmax) of 8.8 micrograms/mL and 17.3 micrograms/mL were obtained approximately 2 h (Tmax), the time of mean peak concentration) after single injections of 2.2 mg/kg and 4.4 mg/kg, respectively. The Cmax was increased approximately twofold following multiple doses of 2.2 mg/kg (Cmax = 13.1 micrograms/mL) and 4.4 mg/kg (Cmax = 24.1 micrograms/mL). Ceftiofur accumulated slowly into TCF and peak concentrations were found to be approximately 14% of those observed in serum after the first dose and approximately 24% after multiple dosing. Concentrations of ceftiofur in BS were obtained rapidly with peak concentrations reaching 45% of the serum Cmax after the first dose. After multiple dosing the Cmax for BS was approximately 25% of the serum Cmax. This study found that both the 2.2 mg/kg and 4.4 mg/kg dosing regimens resulted in continuous serum, TCF and BS concentrations of ceftiofur that exceeded the minimal concentration required to inhibit the bacteria most frequently isolated from calves with acute bovine respiratory disease.     

越桔对长白山区酸性土壤的适应性

在长白山区３种类型的酸性土壤上栽培越桔，研究结果表明，｀北空＇、｀美登＇、｀斯卫克＇在暗棕色森林土上生长结果较好，树体营养关系合理；在草甸沼泽土上３个种生长结果均较差，｀北空＇在水湿地潜育土上生长旺盛，但产量低，综合分析认为，矮丛越桔类型品种对我国长白山区酸性土壤条件的适应性较强，半高丛越桔则适应性差，针对长白山不同类型的土壤，以美登最为适宜暗棕色森林土栽培，斯卫克次之；水湿地潜育土可栽培美登品种     

放牧对鸭茅能量积累及分配的影响

通过长期放牧试验，探讨了不同放牧强度下亚热带高山草地鸭茅种群能量积累规律及能量在不同构件之间的分配特征。在无放牧的对照草地及采食率分别为55％、65％、80％的放牧草地，鸭茅热值随放牧强度的增加而呈单峰曲线型变化，一定强度范围内的放牧干扰提高了鸭茅热值及能量的积累；根部能量的积累随放牧强度的提高而逐渐减少。在禁牧草地，鸭茅种群能量主要被低密度的大分蘖丛所分摊，随着放牧强度的提高，种群能量则逐渐均摊给高密度的小分蘖丛。     

不同氮源对红檬实生苗菌根依赖性的影响

将红檬实生苗培养于改良无土基质中，接种泡囊－丛枝菌根Ｇｌｏｍｕｓｃｉｔｒｏｃｏｌｕｍ，以不接种菌根为对照，并分别施用含氮量相等的３种氮肥，即硝酸按（ＮＯ３－Ｎ）、尿素（ＮＨ２－Ｎ）和硫酸铵（ＮＨ４－Ｎ）。接种菌根１６周后，施不同氮肥的红檬实生苗均有效地感染了菌根，并促进了植株的生长。试验证明，红实生苗施硝态氮（ＮＯ３－Ｎ）使植株的菌根依赖性有明显提高。     

Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.     

Inheritance of myotonic discharges in American quarter horses and the relationship to hyperkalemic periodic paralysis.

Electromyography (EMG) was used to detect myotonic discharges in Quarter Horse breeding stock and to follow the results of mating horses with hyperkalemic periodic paralysis (HPP). The studies were performed on two brood mare farms. A total of six breeding stock showed myotonic discharges and 15 were nonmyotonic. Myotonic discharges were seen in five of six horses belonging to the blood line previously implicated as being predisposed to HPP. Two of these horses had shown clinical signs of HPP. Only one of 15 breeding horses unrelated to the HPP predisposed blood line showed myotonic discharges. When both parents were non-myotonic on EMG than the F1 generation (n = 6) were also nonmyotonic. When a stallion with HPP and myotonic discharges was mated to eight nonmyotonic mares over a six year period half the animals of the F1 generation (n = 25) showed myotonic discharges. When both parents showed myotonic discharges four F1 offspring were myotonic and two were nonmyotonic on EMG testing. There was no evidence of sex linkage. The results are consistent with an autosomal dominant mode of inheritance. Hyperkalemic periodic paralysis and myotonic discharges on EMG may be different manifestations of the same underlying defect.     

剌槐种子萌发过程的微量热法研究

用微量热法测定了剌槐种子的萌发热谱，发现其产热规律与其吸水规律和呼吸规律相关，并获得了衡量种子活力４个参数。